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Flow Cytometry: Early contributions
Cell Functions


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  • Esterases/Phosphatases
    HR Hulett, WA Bonner, J Barrett, LA Herzenberg Cell sorting - Automated separation of mammalian cells as a function of intracellular fluorescence. Science 1969, 166: 747-749.
    G von Sengbusch, C Couwenbergs, J Kühner, U Müller Fluorogenic substrates in single living cells. Histochem J 1976, 8: 341-350.
    ME Wilder, LS Cram Differential fluorochromasia of human lymphocytes as measured by flow cytometry. J Histochem Cytochem 1977, 25: 888-891.
    JV Watson, SH Chambers, P Workman, TS Horsnell Flow cytofluorimetric method for measuring enzyme reaction kinetics in intact cells. FEBS letters 1977, 81: 179-182.
    JV Watson Enzyme kinetic-studies in cell-populations using fluorogenic substrates and flow cytometric techniques. Cytometry 1980, 1:143-151.
    J Malin-Berdel, G Valet Flow cytometric determination of esterases and phosphatase activities and kinetics in hematopoietic cells with fluorogenic substrates. Cytometry 1980, 1: 222-228 (41).
    Beta-glucuronidase
    FA Dolbeare, WF Phares Napthol AS-BI(7-bromo-3-hydroxy-2-naphtho-o-anisidine) phosphatase and naphtol AS-BI beta-d-glucuronidase in chinese-hamster ovary cells - Biochemical and flow cytometric studies. J Histochem Cytochem 1979, 27: 120-124.
    Surface charge density (FITC-polyornithine, polylysine)
    G Valet, S Bamberger G Ruhenstroth-Bauer Flow cytometric determination of surface charge density of the erythrocyte membrane using fluorescinated polycations. J Histochem Cytochem 1979, 27: 342-349 (31).
    Vital and dead cell discrimination (Hoechst 33662/PI)
    M Stöhr, M Vogt-Schaden A dual staining technique for simultaneous flow cytometric DNA analysis of living and dead cells. In: Flow Cytometry IV, Eds: OD Laerum, T Lindmo, E Thorud, Universitetsforlaget, Bergen 1980 p 96-99.
    Intracellular pH (fluorescein diacetate (FDA))
    double excitation fluorescence ratios (cell cluster pH by two measurements)
    JWM Visser, AAM Jongeling, HJ Tanke Intracellular pH determination by fluorescence measurements. J Histochem Cytochem 1979, 27: 32-35.
    Intracellular pH (di-cyanohydrochinone (DCH))
    fluorecence emission ratios (single cell pH)
    G Valet, A Raffael, L Mororder, E Wünsch, G Ruhenstroth-Bauer Fast intracellular pH determination in single cells by flow-cytometry. Naturwiss 1981, 68: 265-266 (44).
    G Valet, G Ruhenstroth-Bauer, E Wünsch, L Moroder Process of determining the pH value in the interior of a cell US Patent 4,677,060 priority Feb 6, 1981 (DE)
    G Valet, G Ruhenstroth-Bauer, E Wünsch, L Moroder Compounds for determining the pH value in the interior of a cell. US Patent 4,515,730 priority Feb 6, 1981 (DE)
    Phagocytosis
    CD Bassoe, J Solsvik, OD Laerum Quantitation of single cell phagocytic capacity by flow cytometry. In: Flow Cytometry IV, Eds: OD Laerum, T Lindmo, E Thorud, Universitetsforlaget, Bergen 1980 p 170-174.
    Intracellular Ca2+ determination (INDO1)
    G Valet, A Raffael Determination of intracellular calcium in vital cells by flow cytometry. Naturwiss 1985, 72: 600-602 (66).
    Respiratory burst 2',7'-dichlorofluorescin (DCFH)
    DA Bass, P Olbrantz, P Szejda, MC Seeds, CE McCall Subpopulations of neutrophils with increased oxidative product formation by neutrophils: A graded response to membrane stimulation. J Immunol 1983, 130: 1910-1917.
    Respiratory burst dihydrorhodamine123 (DHR123)
    G Rothe, A Oser, G Valet Dihydrorhodamine123: A new flow cytometric indicator for respiratory burst activity. Naturwiss 1988, 75: 354-355 (87).
    Respiratory burst O2-indicator hydroethidine (HE)
    G Rothe, G Valet Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2'7'-dichlorofluorescin. J Leu Biol 1990, 47: 440-448 (108).
    Glutathione (orthophtaldialdehyde(OPT))
    J Treumer, G Valet Flow-cytometric determination of glutathione alterations in vital cells by o-phtahldialdehyde. Exp Cell Res 1986, 163: 518-524 (72).
    Glutathione (monobromobimane with N-ethylmaleimide protein thiol group blocking)
    M Poot, A Verkerk, JF Koster, JF Jongkind De novo synthesis of glutathione in human fibroblasts during in-vitro aging and some metabolic diseases as measured by a flow cytometric method BBA 1986, 883: 580-584.
    Intracellular proteinase activites (non viable cells)
    FA Dolbeare, RE Smith Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-beta-naphthylamine. J Clin Chem 1977, 23: 1485-1491.
    Intracellular proteinase activites (R110-substrates in viable cells)
    G Rothe, I Assfalg-Machleidt, W Machleidt, G Valet Independent regulation of endopeptidase activity and respiratory burst activity of neutrophils analyzed by flow cytometry. In: Advances in analytical cellular Pathology, Eds: G Burger, M Oberholzer, GP Voijs, Excerpta Medica, Amsterdam 1990 p 119-120 (101).
    I Assfalg-Machleidt, G Rothe, S Klingel, R Banati, WF Mangel, G Valet, W Machleidt Membrane permeable fluorogenic rhodamine substrates for selective determination of cathepsin L. Biol Chem Hoppe Seyler 1992, 373: 433-440 (121).
    G Rothe, S Klingel, I Assfalg-Machleidt, W Machleidt, C Zirkelbach, RB Banati, WF Mangel, G Valet. Flow cytometric analysis of protease activities in vital cells. Biol Chem Hoppe Seyler 1992, 373: 547-554 (120).
    S Klingel, G Rothe, W Kellermann, G Valet Flow cytometric determination of cysteine and serine proteinase activities in living cells with rhodamine110 substrates. Methods Cell Biol 1994, 41: 449-459 (145).
    S Ganesh, S Klingel, H Kahle, G Valet Flow cytometric determination of aminopeptidase activities in viable cells using fluorogenic rhodamine110 substrates. Cytometry 1995, 20: 334-340 (148).

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    Last Update: Oct 31,2017
    First display: Mar 25,2004